I would stay out of it. Look at it from your future DiL’s point of view. She and her fiancé have made their choices about who will be part of their wedding and now MiL is hoping to persuade them to change these arrangements because she feels it is unfair.
In my experience, men are much less inclined to analyse relationships and worry about perceived slights than women are, and I wonder whether your middle son broached the subject or if you raised it with him because you felt upset on his behalf. When our children are small, we make sure they treat each other fairly, but once they are adults and become partners/spouses/parents they have other loyalties and I don’t think it is wise to try to orchestrate relationships between grown men. If they are close, they will be able to speak frankly to each other without falling out, and if they are not particularly close (or don’t feel strongly about it) then there is no problem.